منابع مشابه
Polyribosomes and protein synthesis in the spleen.
Protein synthesis in rat spleens has been found to occur on polyribosomes. These structures, aggregates of two, three, four, or more ribosomes, become labeled after 1 min of incubation with radioactive amino acids. Incorporation occurs into nascent polypeptide chains which can be released by puromycin or “chased off” by W-amino acids. These polyribosomes stimulate cell-free protein synthesis in...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Techniques in Protein Biosynthesis
The large number and variable quality of recent research papers in the field of protein biosynthesis often has made it difficult for readers to discern clearly the experimental basis (or lack thereof) of the interpretation put on the results that are presented. A new series of volumes has been initiated, therefore, to provide critical discussions of the methods used in this active area of bioch...
متن کاملTechniques in Protein Biosynthesis
This book is volume 2 in a series intended to introduce methods used in the study of protein biosynthesis. The present volume includes chapters on the genetic approach to the study of protein biosynthesis in plant systems, analysis of polysomes, fractionation of ribosomal proteins, animal operative techniques, and the use of sources of high energy phosphate. Inevitably, there is overlap with st...
متن کاملProblems in Protein Biosynthesis
Outline of the steps in protein synthesis. Nature of the genetic code. The use of synthetic oligo- and polynucleotides in deciphering the code. Structure of the code: relatedness of synonym codons. The wobble hypothesis. Chain initiation and N-formyl-methionine. Chain termination and nonsense codons. Mistakes in translation: ambiguity in vitro. Suppressor mutations resulting in ambiguity. Limit...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62257-4